MOV10L1 is necessary for protection of spermatocytes against retrotransposons by Piwi-interacting RNAs. Kim, N. V., Han, J. Other antibodies used were as follows: anti-Vasa (GTX128306; GeneTex, 1:1000), anti-sDMA (SYM11; Millipore, 1:1000), anti-aDMA (ASYM24; Millipore, 1:1000), anti-H3R8me2s (A2374, ABclonal, 1:1000), anti-H4R3me2s (A3159, ABclonal, 1:1000), anti-PRMT5 (A11335, ABclonal, 1:1000), anti-H3(A2348, ABclonal, 1:1000), anti-β-actin (AC004, ABclonal, 1:1000), anti-Flag antibody (F1804, 1:1000 for immunoblot analysis, Sigma-Aldrich) and Alexa Fluor 488 goat anti-rabbit IgG (A11008, 1:1000).Western blotting and co-immunoprecipitation were performed using antibodies mentioned above. Lin, H. & Spradling, A. C. A novel group of pumilio mutations affects the asymmetric division of germline stem cells in the Schmidt, A. et al. We identify a class of 21 nucleotide RNAs, previously named 21U-RNAs, as the piRNAs of C. elegans. All experiments with zebrafish were approved by the Institutional Animal Care and Use Committee of Institute of Hydrobiology, Chinese Academy of Sciences under protocol number 2016-001.The antibodies used were as follows: anti-Ziwi antibody was raised in rabbits against the synthetic peptide, MTGRARARSRGRGRGQE-C; anti-Zili antibody was raised in rabbits against the synthetic peptide, MDPKRPTFPSPPGVIRA-C; and anti-Tdrd1 antibody was raised in rabbits against the synthetic peptide, C-RRPATGPSSLSPRGP.
piRNAs are distinct from miRNAs in several features, the first is size … Kazazian, H. H. Jr. Probing the initiation and effector phases of the somatic piRNA pathway in Olivieri, D., Sykora, M. M., Sachidanandam, R., Mechtler, K. & Brennecke, J.
Kuramochi-Miyagawa, S. et al. The Piwi proteins of the Argonaute superfamily are required for normal germline development in Drosophila, zebrafish, and mice and associate with 24–30 nucleotide RNAs termed piRNAs.
Kirino, Y. et al. Robine, N. et al.
S. & Yokoyama. PIWI-interacting RNAs ... A germline-specific class of small RNAs binds mammalian Piwi proteins. Sign up for the Get the most important science stories of the day, free in your inbox. Find out more in this interview with As the Node celebrates its 10th birthday, we reflect on the decade gone by, recent developments and what’s in store for the future.Enter multiple addresses on separate lines or separate them with commas.This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.State Key Laboratory of Freshwater Ecology and BiotechnologyInstitute of Hydrobiology, Chinese Academy of SciencesState Key Laboratory of Freshwater Ecology and BiotechnologyInstitute of Hydrobiology, Chinese Academy of SciencesState Key Laboratory of Freshwater Ecology and BiotechnologyInstitute of Hydrobiology, Chinese Academy of SciencesState Key Laboratory of Freshwater Ecology and BiotechnologyInstitute of Hydrobiology, Chinese Academy of SciencesState Key Laboratory of Freshwater Ecology and BiotechnologyInstitute of Hydrobiology, Chinese Academy of SciencesState Key Laboratory of Freshwater Ecology and BiotechnologyInstitute of Hydrobiology, Chinese Academy of SciencesState Key Laboratory of Freshwater Ecology and BiotechnologyInstitute of Hydrobiology, Chinese Academy of SciencesState Key Laboratory of Freshwater Ecology and BiotechnologyInstitute of Hydrobiology, Chinese Academy of SciencesState Key Laboratory of Freshwater Ecology and BiotechnologyInstitute of Hydrobiology, Chinese Academy of SciencesState Key Laboratory of Freshwater Ecology and BiotechnologyInstitute of Hydrobiology, Chinese Academy of SciencesInstitute of Hydrobiology, Chinese Academy of SciencesInhibition of Hif1alpha prevents both trauma-induced and genetic heterotopic ossificationBlimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cellsDouble-stranded RNA-mediated silencing of genomic tandem repeats and transposable elements in the D. melanogaster germlineThe methyltransferases PRMT4/CARM1 and PRMT5 control differentially myogenesis in zebrafishMouse Piwi interactome identifies binding mechanism of Tdrkh Tudor domain to arginine methylated MiwiA novel class of evolutionarily conserved genes defined by piwi are essential for stem cell self-renewalpiwi encodes a nucleoplasmic factor whose activity modulates the number and division rate of germline stem cellsmiwi, a murine homolog of piwi, encodes a cytoplasmic protein essential for spermatogenesisMolecular characterisation and inductive expression of a fish protein arginine methyltransferase 1 gene in response to virus infectionSignaling pathways controlling primordial germ cell migration in zebrafishBiogenesis of spliceosomal small nuclear ribonucleoproteinsSynergy between CD52 and CD20 antibody-induced apoptosis correlates with the extent of co-expression of CD20 and CD52 antigens on mononuclear cells derived from chronic lymphocytic leukemia (CLL) and non-Hodgkin's lymphoma (NHL) patients: Implications to antibody-based therapyThe Sm-protein methyltransferase, dart5, is essential for germ-cell specification and maintenanceZebrafish vasa is required for germ-cell differentiation and maintenanceA protein component of Drosophila polar granules is encoded by vasa and has extensive sequence similarity to ATP-dependent helicasesImpaired spermatogenesis, muscle, and erythrocyte function in U12 intron splicing-defective Zrsr1 mutant miceA role for Piwi and piRNAs in germ cell maintenance and transposon silencing in ZebrafishZili is required for germ cell differentiation and meiosis in zebrafishTdrd1 acts as a molecular scaffold for Piwi proteins and piRNA targets in zebrafishIdentification and phylogenetic analyses of the protein arginine methyltransferase gene family in fish and ascidiansInvolvement of the protein of Xenopus vasa homolog (Xenopus vasa-like gene 1, XVLG1) in the differentiation of primordial germ cellsSpatio-temporal expression of Xenopus vasa homolog, XVLG1, in oocytes and embryos: the presence of XVLG1 RNA in somatic cells as well as germline cellsCement dust exposure-related emphysema in a construction workerNuclear m6A reader YTHDC1 regulates alternative polyadenylation and splicing during mouse oocyte developmentPRMT5 protects genomic integrity during global DNA demethylation in primordial germ cells and preimplantation embryosArginine methylation of Piwi proteins catalysed by dPRMT5 is required for Ago3 and Aub stabilityArginine methylation of vasa protein is conserved across phylaIsolation and characterization of a novel gene of the DEAD box protein family which is specifically expressed in germ cells of Xenopus laevisMili, a mammalian member of piwi family gene, is essential for spermatogenesisThe product of the Drosophila gene vasa is very similar to eukaryotic initiation factor-4AHistological and stereological evaluation of zebrafish (Danio rerio) spermatogenesis with an emphasis on spermatogonial generationsThe protein encoded by a murine male germ cell-specific transcript is a putative ATP-dependent RNA helicaseThe Sm protein methyltransferase PRMT5 is not required for primordial germ cell specification in miceLocalization of vasa protein to the Drosophila pole plasm is independent of its RNA-binding and helicase activitiesZebrafish dead end possesses ATPase activity that is required for primordial germ cell developmentZebrafish eaf1 and eaf2/u19 mediate effective convergence and extension movements through the maintenance of wnt11 and wnt5 expressionArginine methyltransferase PRMT5 is essential for sustaining normal adult hematopoiesisForkhead transcription factor 3a (FOXO3a) modulates hypoxia signaling via up-regulation of the von Hippel-Lindau Gene (VHL)Zebrafish foxo3b negatively regulates antiviral response through suppressing the transactivity of irf3 and irf7Morphological development of the gonads in zebrafishAssisted RNP assembly: SMN and PRMT5 complexes cooperate in the formation of spliceosomal UsnRNPsTranslation repression by maternal RNA binding protein Zar1 is essential for early oogenesis in zebrafishFunctional involvement of Tudor and dPRMT5 in the piRNA processing pathway in Drosophila germlinesHuman SWI/SNF-associated PRMT5 methylates histone H3 arginine 8 and negatively regulates expression of ST7 and NM23 tumor suppressor genesImpaired spliceosomal UsnRNP assembly leads to Sm mRNA down-regulation and Sm protein degradationThe function and regulation of vasa-like genes in germ-cell developmentLoss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profileSex Reversal in Zebrafish fancl Mutants Is Caused by Tp53-Mediated Germ Cell ApoptosisEvidence for a piwi-dependent RNA silencing of the gypsy endogenous retrovirus by the Drosophila melanogaster flamenco geneInsulin-like growth factor receptor 1b is required for zebrafish primordial germ cell migration and survivalGerm line control of female sex determination in zebrafishThe PRMT5 arginine methyltransferase: many roles in development, cancer and beyondSox9-related signaling controls zebrafish juvenile ovary-testis transformationPrmt5 is essential for early mouse development and acts in the cytoplasm to maintain ES cell pluripotencySegregation of primordial germ cells: their numbers and fate during early development of Barbus Conchonius (Cyprinidae, Teleostei) as Indicated by 3H-thymidine incorporationThe predominant protein arginine methyltransferase PRMT1 is critical for zebrafish convergence and extension during gastrulationEarly depletion of primordial germ cells in zebrafish promotes testis formationOocyte apoptosis during the transition from ovary-like tissue to testes during sex differentiation of juvenile zebrafishA distinct small RNA pathway silences selfish genetic elements in the germlineProteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family membersProtein arginine methyltransferase 5 (Prmt5) is required for germ cell survival during mouse embryonic developmentPrmt5 is required for germ cell survival during spermatogenesis in miceProtein arginine methylation: a prominent modification and its demethylationThe multiple hats of Vasa: its functions in the germline and in cell cycle progressionZebrafish vasa homologue RNA is localized to the cleavage planes of 2- and 4-cell-stage embryos and is expressed in the primordial germ cellsEffective range of non-cell autonomous activator and inhibitor peptides specifying plant stomatal patterningWRKY55 transcription factor positively regulates leaf senescence and the defense response by modulating the transcription of genes implicated in the biosynthesis of reactive oxygen species and salicylic acid in Loss of U11 small nuclear RNA in the developing mouse limb results in micromeliaWe are aware that the COVID-19 pandemic is having an unprecedented impact on researchers worldwide.
Anne, J.
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